True SVF Cell Isolation
The Microlyzer SVF Kit offers you the perfect system for obtaining a high mesenchymal stem cell population without using enzymes. After harvesting fatty tissue from your patients abdominal area, the tissue is processed through a series of steps to isolate mesenchymal stem cells via SVF techniques. These cells are used to treat non-healing wounds, burns, and serious injuries.
Lipoaspiration
Box 1 - Harvesting Set: Includes all the Lipoaspiration consumables required to harvest fatty tissue from the stomach easily and comfortably. Includes an aspiration syringe with unique locking fins, Single Use Cannula, sterile centrifuge buckets and special decantation apparatus.
Fat Condensing
Box 2 - Fat Condensation Set: In order to begin processing the tissue, the 2nd box contains uniquely adaptive syringes that convert into centrifugation tubes. The fat is centrifuged, separating the Triglyceride, Condensed Fat, and Tumescence.
Micronizing
Box 3 - Micronising Set: After discarding tumescence and triglyceride, the condensed fat is broken up through a series of microlyzers, that break down the connective structure of the mesenchymal stem cells, making them viable for reinjection.
SVF Isolation
Box 4 - SVF Cell Isolation: After a second process of centrifugation, the SVF layer is easily separated from the oil and fat, before being collected using two 10ml PRF tubes and a long needle, Finally, debris is filtered through a final microlyzer and is then ready to inject in conjunction with PRP.
STUDIES AND RESEARCH
Immediately after SVF Cell isolation
Counting and analysis results with a Fluorescent Microscope
[Total cell] : 4.24 x 107 cells/mL
[Nucleated cell] : 24.2 x 106 cells/mL
[Non-nucleated cell] : 1.89 x 107 cells/mL
Viability of nucleated cell : 92.8%
FITC: CD73 : %61.6
APC: CD90 : %81.2
PerCP: CD105 : %2.3
Flow Cytometry was used to differentiate between hematopoietic and endothelial cell populations and the exclusion of surface antigens (CD34, CD29, CD14, CD11b and HLA-DR) helped us to determine the percentage of mesenchymal, endothelial, pericyte, leukocyte, stromal/progenitor cells in the final product.
FITC: CD73 : %61.6
APC: CD90 : %81.2
PerCP: CD105 : %2.3
Flow Cytometry was used to differentiate between hematopoietic and endothelial cell populations and the exclusion of surface antigens (CD34, CD29, CD14, CD11b and HLA-DR) helped us to determine the percentage of mesenchymal, endothelial, pericyte, leukocyte, stromal/progenitor cells in the final product.
BEFORE AND AFTER PHOTOS
One session of the SVF kit.
Before
After
One session of the SVF kit.
Before
After
TRAIN WITH US
BUY NOW
